Test Menu - Transplant Immunology

Test Name TAT Sample Requirement
HLA Typing (High Resolution) A, B, C, DRB1, DQB1 7 days 8 ml EDTA Blood (2 t0 3 Purple Vacutainer)
HLA typing by Luminex (SSOP) A, B, C, DRB1, DQB1 A, B, DRB1 3 Days 8 ml EDTA Blood (2 t0 3 Purple Vacutainer)
Compliment Dependent Cross – Matching (CDC Cross – Matching) Total Lymphocyte Cross – Matching
T-cell Lymphocyte Cross – Matching AHG*
B-cell Lymphocyte Cross – Matching AHG*
Auto Patient’s Cross – Matching
Auto Donor’s Cross – Matching
DTT Treated Serum Cross – Matching
*anti-human globin (AHG) crossmatch
2 Days Recipient – 4ml Plane Tube/ECD Tube Serum Sample (Red Top or Yellow top)
Donor – 18ml (4 to 5 Heparin Green Vacutainer)
Donor Specific Antibody (DSA) 3 Days Recipient – 4ml Plane Tube/ECD Tube Serum Sample (Red Top or Yellow top)
Donor – 18ml (4 to 5 Heparin Green Vacutainer)
Flow Cytometry Cross – Matching T cell Lymphocyte
B cell Lymphocyte
2 Days Recipient – 4ml Plane Tube/RCD Tube Serum Sample (Red Top or Yellow top)
Donor – 18ml (4 to 5 Heparin Green Vacutainer)
HLA Class – I and Class – II  Antibody Screening (Qualitative) 3 Days Recipient – 4ml Plane Tube (Red Top)
Panel Reactive Anitbody (PRA) (Quantitative) Class – I
Class – II
3 Days Recipient – 4ml Plane Tube (Red Top)
Single Antigen Bead Assay (SAB) Class – I Class – II 3 Days Recipient – 4ml Plane Tube (Red Top)
DNA Profiling for Patient and Donor Relationship Establishment (STR Analysis) 5 Days 8ml EDTA Blood (2 to 3 Plane Vacutainer)
Chimerism Analysis (Pre and Post Stem Cell Transplant) 5 Days 8ml EDTA Blood (2 to 3 Plane Vacutainer)
Single Antigen Panel for MICA Antibody (By Luminex) 3 Days Recipient – 4ml Plane Tube (Red Top)
HLA in Disease Association HLA – A 2901/2902 for Birdshot Retinopathy
HLA – B*27 for Ankylosing Spondylitis
HLA – B*51 for Behcet’s Disease
HLA – B*5701 for Abacavir Sensitivity
HLA – B*5801 for Allopurinol Induced Stevens Johnson Syndrome Risk
HLA – DQA 05/DQB1*02, DQA*03/DQB1*0302 for Celiac Disease Risk
HLA – DQB1*0602 for Narcolepsy
HLA – DRB1*1501/1502 for Anti – Glomerular Basement Membrane Disease
HLA*15:02 (Carbamazepine)
3 Days 8ml EDTA Blood (2 to 3 Plane Vacutainer)

Specimen Slims Specimen Type Container Volume Temperature Transport TAT Cut off Status/ Remarks/comments Validation
Whole Blood EDTA, Extracted DNA, Buccal Swab, Dried blood spots (DBS) Whole Blood EDTA, Extracted DNA, Buccal Swab, Dried blood spots (DBS) Purple / Lavender top (3ml)x2 Ambient: 1 hour; Refrigerated: 48 hours;  Frozen: Unacceptable Transport at the earliest after collection in refrigerated condition 40c to 80C (not in frozen) 8 days 5:00 PM Brief history in Organ Transplant Clinical information, Blood transfusion history & Consent form, xerox of ID proof, (both patient & donor) are mandatory. 
Whole Blood EDTA, Extracted DNA, Buccal Swab, Dried blood spots (DBS) and also Blood sample in plain vials Whole Blood EDTA, Extracted DNA, Buccal Swab, Dried blood spots (DBS) and also Blood sample in plain vials Purple / Lavender top     Red/Yellow Purple: (3ml)x2     Red/Yellow: (3ml)x2 Ambient: 1 hour; Refrigerated: 48 hours;  Frozen: Unacceptable Transport at the earliest after collection in refrigerated condition 40c to 80C (not in frozen) 8 days 5:00 PM Brief history in Organ Transplant Clinical information, Blood transfusion history & Consent form, xerox of ID proof, (both patient & donor) are mandatory. 
Whole Blood EDTA, Extracted DNA, Buccal Swab, Dried blood spots (DBS) Whole Blood EDTA, Extracted DNA, Buccal Swab, Dried blood spots (DBS) Purple / Lavender top (3ml)x2 Ambient: 1 hour; Refrigerated: 48 hours;Frozen: Unacceptable Transport at the earliest after collection in refrigerated condition 40c to 80C (not in frozen) 3 days 5:00 PM Brief history in Organ Transplant Clinical information, Blood transfusion history & Consent form, xerox of ID proof, (both patient & donor) are mandatory. 
Whole Blood EDTA, Extracted DNA, Buccal Swab, Dried blood spots (DBS) Whole Blood EDTA, Extracted DNA, Buccal Swab, Dried blood spots (DBS) Purple / Lavender top (3ml)x2 Ambient: 1 hour; Refrigerated: 48 hours;Frozen: Unacceptable Transport at the earliest after collection in refrigerated condition 40c to 80C (not in frozen) 3 days 5:00 PM Brief history in Organ Transplant Clinical information, Blood transfusion history & Consent form is mandatory.  Covered under NABL Scope of Services
Whole Blood EDTA, Extracted DNA, Buccal Swab, Dried blood spots (DBS) Whole Blood EDTA, Extracted DNA, Buccal Swab, Dried blood spots (DBS) Purple / Lavender top (3ml)x2 Ambient: 1 hour; Refrigerated: 48 hours;Frozen: Unacceptable Transport at the earliest after collection in refrigerated condition 40c to 80C (not in frozen) 3 days 5:00 PM Brief history in Organ Transplant Clinical information, Blood transfusion history & Consent form is mandatory.  Covered under NABL Scope of Services
Whole Blood EDTA, Extracted DNA, Buccal Swab, Dried blood spots (DBS) Whole Blood EDTA, Extracted DNA, Buccal Swab, Dried blood spots (DBS) Purple / Lavender top (3ml)x2 Ambient: 1 hour; Refrigerated: 48 hours;Frozen: Unacceptable Transport at the earliest after collection in refrigerated condition 40c to 80C (not in frozen) 3 days 5:00 PM Brief history in Organ Transplant Clinical information, Blood transfusion history & Consent form is mandatory.  Covered under NABL Scope of Services
Whole Blood EDTA, Extracted DNA, Buccal Swab, Dried blood spots (DBS) Whole Blood EDTA, Extracted DNA, Buccal Swab, Dried blood spots (DBS) Purple / Lavender top (3ml)x2 Ambient: 1 hour; Refrigerated: 48 hours;Frozen: Unacceptable Transport at the earliest after collection in refrigerated condition 40c to 80C (not in frozen) 3 days 5.00 PM Blood transfusion history & Consent form is mandatory. Internal validation done with regular proficiency testing and due deligenece
Whole Blood EDTA, Extracted DNA, Buccal Swab, Dried blood spots (DBS) Whole Blood EDTA, Extracted DNA, Buccal Swab, Dried blood spots (DBS) Purple / Lavender top (3ml)x2 Ambient: 1 hour; Refrigerated: 48 hours;Frozen: Unacceptable Transport at the earliest after collection in refrigerated condition 40c to 80C (not in frozen) 3 days 5:00 PM Brief history in Organ Transplant Clinical information, Blood transfusion history & Consent form is mandatory.  Covered under NABL Scope of Services
Whole Blood EDTA, Extracted DNA, Buccal Swab, Dried blood spots (DBS) Whole Blood EDTA, Extracted DNA, Buccal Swab, Dried blood spots (DBS) Purple / Lavender top (3ml)x2 Ambient: 1 hour; Refrigerated: 48 hours;Frozen: Unacceptable Transport at the earliest after collection in refrigerated condition 40c to 80C (not in frozen) 3 days 5:00 PM Brief history in Organ Transplant Clinical information, Blood transfusion history & Consent form is mandatory.  Covered under NABL Scope of Services
Whole Blood EDTA, Extracted DNA, Buccal Swab, Dried blood spots (DBS) Whole Blood EDTA, Extracted DNA, Buccal Swab, Dried blood spots (DBS) Purple / Lavender top (3ml)x2 Ambient: 1 hour; Refrigerated: 48 hours;Frozen: Unacceptable Transport at the earliest after collection in refrigerated condition 40c to 80C (not in frozen) 8 days 5:00 PM Brief history in Organ Transplant Clinical information, Blood transfusion history & Consent form is mandatory.  Covered under NABL Scope of Services
Whole Blood EDTA, Extracted DNA, Buccal Swab, Dried blood spots (DBS) Whole Blood EDTA, Extracted DNA, Buccal Swab, Dried blood spots (DBS) Purple / Lavender top (3ml)x2 Ambient: 1 hour; Refrigerated: 48 hours;Frozen: Unacceptable Transport at the earliest after collection in refrigerated condition 40c to 80C (not in frozen) 3 days 5:00 PM
Whole Blood EDTA, Extracted DNA, Buccal Swab, Dried blood spots (DBS) Whole Blood EDTA, Extracted DNA, Buccal Swab, Dried blood spots (DBS) Purple / Lavender top (3ml)x2 Ambient: 1 hour; Refrigerated: 48 hours;Frozen: Unacceptable Transport at the earliest after collection in refrigerated condition 40c to 80C (not in frozen) 3 days 5:00 PM
Whole Blood EDTA, Extracted DNA, Buccal Swab, Dried blood spots (DBS) Whole Blood EDTA, Extracted DNA, Buccal Swab, Dried blood spots (DBS) Purple / Lavender top (3ml)x2 Ambient: 1 hour; Refrigerated: 48 hours;Frozen: Unacceptable Transport at the earliest after collection in refrigerated condition 40c to 80C (not in frozen) 3 days 5:00 PM
Whole Blood EDTA, Extracted DNA, Buccal Swab, Dried blood spots (DBS) Whole Blood EDTA, Extracted DNA, Buccal Swab, Dried blood spots (DBS) Purple / Lavender top (3ml)x2 Ambient: 1 hour; Refrigerated: 48 hours;Frozen: Unacceptable Transport at the earliest after collection in refrigerated condition 40c to 80C (not in frozen) 8 days 5:00 PM
Donor: Blood in Heparin vials Recipient: Plain vials Donor: Blood in Heparin vials Recipient: Plain vials Donor: Green   Recipient: Red/Yellow Donor:(3ml)x4       Recipient: (3ml) x2 Ambient: 1 hour; Refrigerated: 48 hours;Frozen: Unacceptable Transport at the earliest after collection in refrigerated condition 40c to 80C (not in frozen) 3 days 5:00 PM Brief history in Organ Transplant Clinical information, Blood transfusion history & Consent form is mandatory.  Covered under NABL Scope of Services
Donor: Blood in Heparin vials Recipient: Plain vials Donor: Blood in Heparin vials Recipient: Plain vials Donor: Green   Recipient: Red/Yellow Donor: (3ml)x4  Recipient: (3ml)x2 Ambient: 1 hour; Refrigerated: 48 hours;Frozen: Unacceptable Transport at the earliest after collection in refrigerated condition 40c to 80C (not in frozen) 3 days 5:00 PM Test Requisition form (TRF) is mandatory Internal validation done with regular proficiency testing and due deligenece
Donor: Blood in Heparin and Plain vials Donor: Blood in Heparin and Plain vials Donor: Green, Red/Yellow Donor: (3ml)x4  and (3ml)x2 Ambient: 1 hour; Refrigerated: 48 hours;Frozen: Unacceptable Transport at the earliest after collection in refrigerated condition 40c to 80C (not in frozen) 3 days 5:00 PM Test Requisition form (TRF) is mandatory Internal validation done with regular proficiency testing and due deligenece
Recipient: Blood in Heparin and Plain vials Recipient: Blood in Heparin and Plain vials Recipient: Green, Red/Yellow Recipient: (3ml)x4  and (3ml)x2 Ambient: 1 hour; Refrigerated: 48 hours;Frozen: Unacceptable Transport at the earliest after collection in refrigerated condition 40c to 80C (not in frozen) 3 days 5:00 PM Test Requisition form (TRF) is mandatory Internal validation done with regular proficiency testing and due deligenece
Donor: Blood in Heparin vials Recipient: Plain vials Donor: Blood in Heparin vials Recipient: Plain vials Donor: Green   Recipient: Red/Yellow Donor: (3ml)x4  Recipient: (3ml)x2 Ambient: 1 hour; Refrigerated: 48 hours;Frozen: Unacceptable Transport at the earliest after collection in refrigerated condition 40c to 80C (not in frozen) 3 days 5:00 PM Test Requisition form (TRF) is mandatory Internal validation done with regular proficiency testing and due deligenece
Recipient: Plain vials Recipient: Plain vials Recipient: Red/Yellow (3ml)x2 Ambient: 1 hour; Refrigerated: 48 hours;Frozen: Unacceptable Transport at the earliest after collection in refrigerated condition 40c to 80C (not in frozen) 3 days 5:00 PM Blood transfusion history & Consent form is mandatory. Internal validation done with regular proficiency testing and due deligenece
Recipient: Plain vials Recipient: Plain vials Recipient: Red/Yellow (3ml)x2 Ambient: 1 hour; Refrigerated: 48 hours;Frozen: Unacceptable Transport at the earliest after collection in refrigerated condition 40c to 80C (not in frozen) 3 days 5:00 PM Blood transfusion history & Consent form is mandatory. Internal validation done with regular proficiency testing and due deligenece
Recipient: Plain vials Recipient: Plain vials Recipient: Red/Yellow (3ml)x2 Ambient: 1 hour; Refrigerated: 48 hours;Frozen: Unacceptable Transport at the earliest after collection in refrigerated condition 40c to 80C (not in frozen) 3days 5:00 PM Blood transfusion history & Consent form is mandatory. Internal validation done with regular proficiency testing and due deligenece
Recipient: Plain vials Recipient: Plain vials Recipient: Red/Yellow (3ml)x2 Ambient: 1 hour; Refrigerated: 48 hours;Frozen: Unacceptable Transport at the earliest after collection in refrigerated condition 40c to 80C (not in frozen) 3 days 5:00 PM Blood transfusion history & Consent form is mandatory. Internal validation done with regular proficiency testing and due deligenece
Recipient: Plain vials Recipient: Plain vials Recipient: Red/Yellow (3ml)x2 Ambient: 1 hour; Refrigerated: 48 hours;Frozen: Unacceptable Transport at the earliest after collection in refrigerated condition 40c to 80C (not in frozen) 3 days 5:00 PM Blood transfusion history & Consent form is mandatory. Internal validation done with regular proficiency testing and due deligenece
Recipient: Plain vials Recipient: Plain vials Red/Yellow (3ml)x2 Ambient: 1 hour; Refrigerated: 48 hours;Frozen: Unacceptable Transport at the earliest after collection in refrigerated condition 40c to 80C (not in frozen) 3 days 5:00 PM Blood transfusion history & Consent form is mandatory. Internal validation done with regular proficiency testing and due deligenece
Donor: Blood in Heparin vials Recipient: Plain vials Donor: Blood in Heparin vials Recipient: Plain vials Red/Yellow (3ml)x2 Ambient: 1 hour; Refrigerated: 48 hours;Frozen: Unacceptable Transport at the earliest after collection in refrigerated condition 40c to 80C (not in frozen) 3 days 5:00 PM Test Requisition form (TRF) is mandatory Internal validation done with regular proficiency testing and due deligenece
Whole Blood EDTA Whole Blood EDTA Purple / Lavender top (3ml)x2 Ambient: 1 hour; Refrigerated: 48 hours;Frozen: Unacceptable Transport at the earliest after collection in refrigerated condition 40c to 80C (not in frozen) 8 days 5:00 PM Brief history in Organ Transplant Clinical information, Blood transfusion history & Consent form is mandatory.  Covered under NABL Scope of Services
Whole Blood EDTA Whole Blood EDTA Purple / Lavender top (3ml)x2 Ambient: 1 hour; Refrigerated: 48 hours;Frozen: Unacceptable Transport at the earliest after collection in refrigerated condition 40c to 80C (not in frozen) 8 days 5:00 PM Brief history in Organ Transplant Clinical information, Blood transfusion history & Consent form is mandatory.  Covered under NABL Scope of Services
Whole Blood EDTA Whole Blood EDTA Purple / Lavender top (3ml)x2 Ambient: 1 hour; Refrigerated: 48 hours;Frozen: Unacceptable Transport at the earliest after collection in refrigerated condition 40c to 80C (not in frozen) 3 days 5:00 PM Internal validation done with regular proficiency testing and due deligenece

Accreditations

CLIA No. 34D2205781