Test Menu - Transplant Immunology
Test Name | TAT | Sample Requirement | |
---|---|---|---|
HLA Typing (High Resolution) | A, B, C, DRB1, DQB1 | 7 days | 8 ml EDTA Blood (2 t0 3 Purple Vacutainer) |
HLA typing by Luminex (SSOP) | A, B, C, DRB1, DQB1 A, B, DRB1 | 3 Days | 8 ml EDTA Blood (2 t0 3 Purple Vacutainer) |
Compliment Dependent Cross – Matching (CDC Cross – Matching) | Total Lymphocyte Cross – Matching T-cell Lymphocyte Cross – Matching AHG* B-cell Lymphocyte Cross – Matching AHG* Auto Patient’s Cross – Matching Auto Donor’s Cross – Matching DTT Treated Serum Cross – Matching *anti-human globin (AHG) crossmatch |
2 Days | Recipient – 4ml Plane Tube/ECD Tube Serum Sample (Red Top or Yellow top) Donor – 18ml (4 to 5 Heparin Green Vacutainer) |
Donor Specific Antibody (DSA) | 3 Days | Recipient – 4ml Plane Tube/ECD Tube Serum Sample (Red Top or Yellow top) Donor – 18ml (4 to 5 Heparin Green Vacutainer) |
|
Flow Cytometry Cross – Matching | T cell Lymphocyte B cell Lymphocyte |
2 Days | Recipient – 4ml Plane Tube/RCD Tube Serum Sample (Red Top or Yellow top) Donor – 18ml (4 to 5 Heparin Green Vacutainer) |
HLA Class – I and Class – II Antibody Screening (Qualitative) | 3 Days | Recipient – 4ml Plane Tube (Red Top) | |
Panel Reactive Anitbody (PRA) (Quantitative) | Class – I Class – II |
3 Days | Recipient – 4ml Plane Tube (Red Top) |
Single Antigen Bead Assay (SAB) | Class – I Class – II | 3 Days | Recipient – 4ml Plane Tube (Red Top) |
DNA Profiling for Patient and Donor Relationship Establishment (STR Analysis) | 5 Days | 8ml EDTA Blood (2 to 3 Plane Vacutainer) | |
Chimerism Analysis (Pre and Post Stem Cell Transplant) | 5 Days | 8ml EDTA Blood (2 to 3 Plane Vacutainer) | |
Single Antigen Panel for MICA Antibody (By Luminex) | 3 Days | Recipient – 4ml Plane Tube (Red Top) | |
HLA in Disease Association | HLA – A 2901/2902 for Birdshot Retinopathy HLA – B*27 for Ankylosing Spondylitis HLA – B*51 for Behcet’s Disease HLA – B*5701 for Abacavir Sensitivity HLA – B*5801 for Allopurinol Induced Stevens Johnson Syndrome Risk HLA – DQA 05/DQB1*02, DQA*03/DQB1*0302 for Celiac Disease Risk HLA – DQB1*0602 for Narcolepsy HLA – DRB1*1501/1502 for Anti – Glomerular Basement Membrane Disease HLA*15:02 (Carbamazepine) |
3 Days | 8ml EDTA Blood (2 to 3 Plane Vacutainer) |
Specimen Slims | Specimen Type | Container | Volume | Temperature | Transport | TAT | Cut off | Status/ Remarks/comments | Validation |
---|---|---|---|---|---|---|---|---|---|
Whole Blood EDTA, Extracted DNA, Buccal Swab, Dried blood spots (DBS) | Whole Blood EDTA, Extracted DNA, Buccal Swab, Dried blood spots (DBS) | Purple / Lavender top | (3ml)x2 | Ambient: 1 hour; Refrigerated: 48 hours; Frozen: Unacceptable | Transport at the earliest after collection in refrigerated condition 40c to 80C (not in frozen) | 8 days | 5:00 PM | Brief history in Organ Transplant Clinical information, Blood transfusion history & Consent form, xerox of ID proof, (both patient & donor) are mandatory. | |
Whole Blood EDTA, Extracted DNA, Buccal Swab, Dried blood spots (DBS) and also Blood sample in plain vials | Whole Blood EDTA, Extracted DNA, Buccal Swab, Dried blood spots (DBS) and also Blood sample in plain vials | Purple / Lavender top Red/Yellow | Purple: (3ml)x2 Red/Yellow: (3ml)x2 | Ambient: 1 hour; Refrigerated: 48 hours; Frozen: Unacceptable | Transport at the earliest after collection in refrigerated condition 40c to 80C (not in frozen) | 8 days | 5:00 PM | Brief history in Organ Transplant Clinical information, Blood transfusion history & Consent form, xerox of ID proof, (both patient & donor) are mandatory. | |
Whole Blood EDTA, Extracted DNA, Buccal Swab, Dried blood spots (DBS) | Whole Blood EDTA, Extracted DNA, Buccal Swab, Dried blood spots (DBS) | Purple / Lavender top | (3ml)x2 | Ambient: 1 hour; Refrigerated: 48 hours;Frozen: Unacceptable | Transport at the earliest after collection in refrigerated condition 40c to 80C (not in frozen) | 3 days | 5:00 PM | Brief history in Organ Transplant Clinical information, Blood transfusion history & Consent form, xerox of ID proof, (both patient & donor) are mandatory. | |
Whole Blood EDTA, Extracted DNA, Buccal Swab, Dried blood spots (DBS) | Whole Blood EDTA, Extracted DNA, Buccal Swab, Dried blood spots (DBS) | Purple / Lavender top | (3ml)x2 | Ambient: 1 hour; Refrigerated: 48 hours;Frozen: Unacceptable | Transport at the earliest after collection in refrigerated condition 40c to 80C (not in frozen) | 3 days | 5:00 PM | Brief history in Organ Transplant Clinical information, Blood transfusion history & Consent form is mandatory. | Covered under NABL Scope of Services |
Whole Blood EDTA, Extracted DNA, Buccal Swab, Dried blood spots (DBS) | Whole Blood EDTA, Extracted DNA, Buccal Swab, Dried blood spots (DBS) | Purple / Lavender top | (3ml)x2 | Ambient: 1 hour; Refrigerated: 48 hours;Frozen: Unacceptable | Transport at the earliest after collection in refrigerated condition 40c to 80C (not in frozen) | 3 days | 5:00 PM | Brief history in Organ Transplant Clinical information, Blood transfusion history & Consent form is mandatory. | Covered under NABL Scope of Services |
Whole Blood EDTA, Extracted DNA, Buccal Swab, Dried blood spots (DBS) | Whole Blood EDTA, Extracted DNA, Buccal Swab, Dried blood spots (DBS) | Purple / Lavender top | (3ml)x2 | Ambient: 1 hour; Refrigerated: 48 hours;Frozen: Unacceptable | Transport at the earliest after collection in refrigerated condition 40c to 80C (not in frozen) | 3 days | 5:00 PM | Brief history in Organ Transplant Clinical information, Blood transfusion history & Consent form is mandatory. | Covered under NABL Scope of Services |
Whole Blood EDTA, Extracted DNA, Buccal Swab, Dried blood spots (DBS) | Whole Blood EDTA, Extracted DNA, Buccal Swab, Dried blood spots (DBS) | Purple / Lavender top | (3ml)x2 | Ambient: 1 hour; Refrigerated: 48 hours;Frozen: Unacceptable | Transport at the earliest after collection in refrigerated condition 40c to 80C (not in frozen) | 3 days | 5.00 PM | Blood transfusion history & Consent form is mandatory. | Internal validation done with regular proficiency testing and due deligenece |
Whole Blood EDTA, Extracted DNA, Buccal Swab, Dried blood spots (DBS) | Whole Blood EDTA, Extracted DNA, Buccal Swab, Dried blood spots (DBS) | Purple / Lavender top | (3ml)x2 | Ambient: 1 hour; Refrigerated: 48 hours;Frozen: Unacceptable | Transport at the earliest after collection in refrigerated condition 40c to 80C (not in frozen) | 3 days | 5:00 PM | Brief history in Organ Transplant Clinical information, Blood transfusion history & Consent form is mandatory. | Covered under NABL Scope of Services |
Whole Blood EDTA, Extracted DNA, Buccal Swab, Dried blood spots (DBS) | Whole Blood EDTA, Extracted DNA, Buccal Swab, Dried blood spots (DBS) | Purple / Lavender top | (3ml)x2 | Ambient: 1 hour; Refrigerated: 48 hours;Frozen: Unacceptable | Transport at the earliest after collection in refrigerated condition 40c to 80C (not in frozen) | 3 days | 5:00 PM | Brief history in Organ Transplant Clinical information, Blood transfusion history & Consent form is mandatory. | Covered under NABL Scope of Services |
Whole Blood EDTA, Extracted DNA, Buccal Swab, Dried blood spots (DBS) | Whole Blood EDTA, Extracted DNA, Buccal Swab, Dried blood spots (DBS) | Purple / Lavender top | (3ml)x2 | Ambient: 1 hour; Refrigerated: 48 hours;Frozen: Unacceptable | Transport at the earliest after collection in refrigerated condition 40c to 80C (not in frozen) | 8 days | 5:00 PM | Brief history in Organ Transplant Clinical information, Blood transfusion history & Consent form is mandatory. | Covered under NABL Scope of Services |
Whole Blood EDTA, Extracted DNA, Buccal Swab, Dried blood spots (DBS) | Whole Blood EDTA, Extracted DNA, Buccal Swab, Dried blood spots (DBS) | Purple / Lavender top | (3ml)x2 | Ambient: 1 hour; Refrigerated: 48 hours;Frozen: Unacceptable | Transport at the earliest after collection in refrigerated condition 40c to 80C (not in frozen) | 3 days | 5:00 PM | ||
Whole Blood EDTA, Extracted DNA, Buccal Swab, Dried blood spots (DBS) | Whole Blood EDTA, Extracted DNA, Buccal Swab, Dried blood spots (DBS) | Purple / Lavender top | (3ml)x2 | Ambient: 1 hour; Refrigerated: 48 hours;Frozen: Unacceptable | Transport at the earliest after collection in refrigerated condition 40c to 80C (not in frozen) | 3 days | 5:00 PM | ||
Whole Blood EDTA, Extracted DNA, Buccal Swab, Dried blood spots (DBS) | Whole Blood EDTA, Extracted DNA, Buccal Swab, Dried blood spots (DBS) | Purple / Lavender top | (3ml)x2 | Ambient: 1 hour; Refrigerated: 48 hours;Frozen: Unacceptable | Transport at the earliest after collection in refrigerated condition 40c to 80C (not in frozen) | 3 days | 5:00 PM | ||
Whole Blood EDTA, Extracted DNA, Buccal Swab, Dried blood spots (DBS) | Whole Blood EDTA, Extracted DNA, Buccal Swab, Dried blood spots (DBS) | Purple / Lavender top | (3ml)x2 | Ambient: 1 hour; Refrigerated: 48 hours;Frozen: Unacceptable | Transport at the earliest after collection in refrigerated condition 40c to 80C (not in frozen) | 8 days | 5:00 PM | ||
Donor: Blood in Heparin vials Recipient: Plain vials | Donor: Blood in Heparin vials Recipient: Plain vials | Donor: Green Recipient: Red/Yellow | Donor:(3ml)x4 Recipient: (3ml) x2 | Ambient: 1 hour; Refrigerated: 48 hours;Frozen: Unacceptable | Transport at the earliest after collection in refrigerated condition 40c to 80C (not in frozen) | 3 days | 5:00 PM | Brief history in Organ Transplant Clinical information, Blood transfusion history & Consent form is mandatory. | Covered under NABL Scope of Services |
Donor: Blood in Heparin vials Recipient: Plain vials | Donor: Blood in Heparin vials Recipient: Plain vials | Donor: Green Recipient: Red/Yellow | Donor: (3ml)x4 Recipient: (3ml)x2 | Ambient: 1 hour; Refrigerated: 48 hours;Frozen: Unacceptable | Transport at the earliest after collection in refrigerated condition 40c to 80C (not in frozen) | 3 days | 5:00 PM | Test Requisition form (TRF) is mandatory | Internal validation done with regular proficiency testing and due deligenece |
Donor: Blood in Heparin and Plain vials | Donor: Blood in Heparin and Plain vials | Donor: Green, Red/Yellow | Donor: (3ml)x4 and (3ml)x2 | Ambient: 1 hour; Refrigerated: 48 hours;Frozen: Unacceptable | Transport at the earliest after collection in refrigerated condition 40c to 80C (not in frozen) | 3 days | 5:00 PM | Test Requisition form (TRF) is mandatory | Internal validation done with regular proficiency testing and due deligenece |
Recipient: Blood in Heparin and Plain vials | Recipient: Blood in Heparin and Plain vials | Recipient: Green, Red/Yellow | Recipient: (3ml)x4 and (3ml)x2 | Ambient: 1 hour; Refrigerated: 48 hours;Frozen: Unacceptable | Transport at the earliest after collection in refrigerated condition 40c to 80C (not in frozen) | 3 days | 5:00 PM | Test Requisition form (TRF) is mandatory | Internal validation done with regular proficiency testing and due deligenece |
Donor: Blood in Heparin vials Recipient: Plain vials | Donor: Blood in Heparin vials Recipient: Plain vials | Donor: Green Recipient: Red/Yellow | Donor: (3ml)x4 Recipient: (3ml)x2 | Ambient: 1 hour; Refrigerated: 48 hours;Frozen: Unacceptable | Transport at the earliest after collection in refrigerated condition 40c to 80C (not in frozen) | 3 days | 5:00 PM | Test Requisition form (TRF) is mandatory | Internal validation done with regular proficiency testing and due deligenece |
Recipient: Plain vials | Recipient: Plain vials | Recipient: Red/Yellow | (3ml)x2 | Ambient: 1 hour; Refrigerated: 48 hours;Frozen: Unacceptable | Transport at the earliest after collection in refrigerated condition 40c to 80C (not in frozen) | 3 days | 5:00 PM | Blood transfusion history & Consent form is mandatory. | Internal validation done with regular proficiency testing and due deligenece |
Recipient: Plain vials | Recipient: Plain vials | Recipient: Red/Yellow | (3ml)x2 | Ambient: 1 hour; Refrigerated: 48 hours;Frozen: Unacceptable | Transport at the earliest after collection in refrigerated condition 40c to 80C (not in frozen) | 3 days | 5:00 PM | Blood transfusion history & Consent form is mandatory. | Internal validation done with regular proficiency testing and due deligenece |
Recipient: Plain vials | Recipient: Plain vials | Recipient: Red/Yellow | (3ml)x2 | Ambient: 1 hour; Refrigerated: 48 hours;Frozen: Unacceptable | Transport at the earliest after collection in refrigerated condition 40c to 80C (not in frozen) | 3days | 5:00 PM | Blood transfusion history & Consent form is mandatory. | Internal validation done with regular proficiency testing and due deligenece |
Recipient: Plain vials | Recipient: Plain vials | Recipient: Red/Yellow | (3ml)x2 | Ambient: 1 hour; Refrigerated: 48 hours;Frozen: Unacceptable | Transport at the earliest after collection in refrigerated condition 40c to 80C (not in frozen) | 3 days | 5:00 PM | Blood transfusion history & Consent form is mandatory. | Internal validation done with regular proficiency testing and due deligenece |
Recipient: Plain vials | Recipient: Plain vials | Recipient: Red/Yellow | (3ml)x2 | Ambient: 1 hour; Refrigerated: 48 hours;Frozen: Unacceptable | Transport at the earliest after collection in refrigerated condition 40c to 80C (not in frozen) | 3 days | 5:00 PM | Blood transfusion history & Consent form is mandatory. | Internal validation done with regular proficiency testing and due deligenece |
Recipient: Plain vials | Recipient: Plain vials | Red/Yellow | (3ml)x2 | Ambient: 1 hour; Refrigerated: 48 hours;Frozen: Unacceptable | Transport at the earliest after collection in refrigerated condition 40c to 80C (not in frozen) | 3 days | 5:00 PM | Blood transfusion history & Consent form is mandatory. | Internal validation done with regular proficiency testing and due deligenece |
Donor: Blood in Heparin vials Recipient: Plain vials | Donor: Blood in Heparin vials Recipient: Plain vials | Red/Yellow | (3ml)x2 | Ambient: 1 hour; Refrigerated: 48 hours;Frozen: Unacceptable | Transport at the earliest after collection in refrigerated condition 40c to 80C (not in frozen) | 3 days | 5:00 PM | Test Requisition form (TRF) is mandatory | Internal validation done with regular proficiency testing and due deligenece |
Whole Blood EDTA | Whole Blood EDTA | Purple / Lavender top | (3ml)x2 | Ambient: 1 hour; Refrigerated: 48 hours;Frozen: Unacceptable | Transport at the earliest after collection in refrigerated condition 40c to 80C (not in frozen) | 8 days | 5:00 PM | Brief history in Organ Transplant Clinical information, Blood transfusion history & Consent form is mandatory. | Covered under NABL Scope of Services |
Whole Blood EDTA | Whole Blood EDTA | Purple / Lavender top | (3ml)x2 | Ambient: 1 hour; Refrigerated: 48 hours;Frozen: Unacceptable | Transport at the earliest after collection in refrigerated condition 40c to 80C (not in frozen) | 8 days | 5:00 PM | Brief history in Organ Transplant Clinical information, Blood transfusion history & Consent form is mandatory. | Covered under NABL Scope of Services |
Whole Blood EDTA | Whole Blood EDTA | Purple / Lavender top | (3ml)x2 | Ambient: 1 hour; Refrigerated: 48 hours;Frozen: Unacceptable | Transport at the earliest after collection in refrigerated condition 40c to 80C (not in frozen) | 3 days | 5:00 PM | Internal validation done with regular proficiency testing and due deligenece |